Dear Loreto,

unlike for p-tyrosine, there are no single antibodies that will recognize all or most p-serines or p-threonines independent of the surrounding amino acid sequence. The epitope for a phosphorylated tyrosine is very specific, but phosphorylated serines and threonines interact much stronger with their environment and thus don't create specific epitopes. You will have to test the different antibodies out their for your specific needs and maybe work with a mix of antibodies depending on your question. Hope this helps.

Dear Loreto,

unlike for p-tyrosine, there are no single antibodies that will recognize all or most p-serines or p-threonines independent of the surrounding amino acid sequence. The epitope for a phosphorylated tyrosine is very specific, but phosphorylated serines and threonines interact much stronger with their environment and thus don't create specific epitopes. You will have to test the different antibodies out their for your specific needs and maybe work with a mix of antibodies depending on your question. Hope this helps.

Thanks!!!! I am studying some phosphorylation buy unfortunately don´t exist specifics antibodies (commercial) for these proteins. Do you know any antibodies? or Do you think that radioactivity is a better alternative? Thanks again.

Loreto, check out Cell Signaling, http://www.cellsignal.com/products/9631.html, also The more specific antibodies on the same page further down. See also Millipore, eg. http://www.millipore.com/catalogue/item/ab1603 or http://www.millipore.com/catalogue/item/ab1607. Also Abcam and Qiagene has similar antibodies, see what is working for you.

Thanks a lot!!!!!! :)

we frequently use this one, which works pretty fine:

p-Threonine Cell Signaling #9381 rabbit

Thanks a lot everybody!!!! :)

I would recommend Abcam for Western Blotting and CellSignaling Technologies for IP

Hi everyone ! One could alternatively try a phos-tag resin that sems to work for some proteins ate least in pull-down or band shift assay when added directly to the gel !

good luck

Phos-tag resin could be used but it depends the type of proteins