Check out the in silico PCR from the UCSC website, I use it for all my primer designs: http://genome.ucsc.edu/cgi-bin/hgPcr?command=start

Check out the in silico PCR from the UCSC website, I use it for all my primer designs: http://genome.ucsc.edu/cgi-bin/hgPcr?command=start

I use: http://biocompute.bmi.ac.cn/CZlab/MFEprimer-2.0/

with great results.

SerialCloner has a in silico PCR tool, and is also great for cloning, RE maps, etcetera. Runs on your desk top. I liked it so much I donated to SerialBasics.

http://serialbasics.free.fr/Serial_Cloner-Download.html

Try snapgene. They have a 30 day free trial

Try http://insilico.ehu.es/

Thank you each one of you

thanks

Thanks every one

A website I use frequently is BiSearch: bisearch.enzim.hu. Simple, free and does the job.

References:

Tusnády GE, Simon I, Váradi A and Arányi T (2005)

BiSearch: Primer-design and Search Tool for PCR on Bisulfite Treated Genomes.

Nucleic Acids Research 33, e9.

Arányi T, Váradi A, Simon I and Tusnády GE (2006)

The BiSearch web server.

BMC Bioinformatics 7, 431.

Thank you all

for insilico PCR refer to  http://insilico.ehu.es/PCR/

you can also provide the species sequence against which PCR to be performed

Fastpcr tool

you can also use primerblast in ncbi.

http://insilico.ehu.es/PCR/

Does anybody suggested a paper used insilico to perform his work?