The concentration of a compound for a given xenograft differs significantly based on what cells are used. Even for hepatocellular xenografts, the differing growth curves (http://altogenlabs.com/xenograft-models/liver-cancer-xenograft/) are already enough to warrant different concentrations. That's not even mentioning the route of delivery (injection, oral, etc.) and the location of delivery (subcutaneous, intratumorial, etc.), which also impact the concentration. The previous answers have been around right (5 mg/kg is good) but obviously previous literature and experimentation should determine final concentrations.

I guess 30 mg/kg is too high. It might show excessive toxicity. 2.5 and 5 is rational in term of use in human.

Thank you Dr.Ference Rick for your valuable suggestion and article

Thank you Mr. Nurunnabi for your suggestion

Hi We used the Clinical Dose of 5FU for xenograft work on SCID mice, which is 12mg/kg. We gave i.p. injections 1 x weekly, for 2 - 3 weeks.

Thank you Karen

Our team used 5 mg/kg daily (oral route) in Balb/c mice for 4 consecutive weeks, gave very good result. All the best to your research

Thank you Dr. Heshu

The concentration of a compound for a given xenograft differs significantly based on what cells are used. Even for hepatocellular xenografts, the differing growth curves (http://altogenlabs.com/xenograft-models/liver-cancer-xenograft/) are already enough to warrant different concentrations. That's not even mentioning the route of delivery (injection, oral, etc.) and the location of delivery (subcutaneous, intratumorial, etc.), which also impact the concentration. The previous answers have been around right (5 mg/kg is good) but obviously previous literature and experimentation should determine final concentrations.