RNA extraction from exocrine pancreas?

Hi everyone ! Does anyone have any experience in extracting RNA from exocrine pancreas (which is very high in RNAse)? The pancreas is frozen tissue. I wonder if RNAse is also high in the nucleus,...

31 January 2021 390 2 View

Cell culture Vitamin D free media ???

Dear researchers, I am working onn the influence of vitamin D on cancer. I was wondering whether ther is Vitamin D free media , since I wanna be completly sure of my negative control without...

01 November 2020 582 2 View

Western blot , Blocking step milk vs BSA ?

concerning the westrn blot ..........I am diluting my antibodies in 5% bsa , but I get very high backgroud though I an diluting the secondry antibody in 5% milk . My question is . Can I block the...

13 April 2020 297 4 View

What is benefits of NN in the green building design and operation?

Green building is the practice of creating structures and using processes that are environmentally responsible and resource-efficient throughout a building's life-cycle from siting to design,...

09 September 2018 2,082 2 View

Please , ..what are the applications an image recognition in real time medical systems?

i have related research for this way, i looking for new approach....

26 December 2017 5,011 2 View

How is converting hypoxic tumor cells to normoxic tumor cells?

hypoxic tumor metabolitic enzymes

14 March 2017 7,228 3 View

Can anyone suggest a software to organize experiments from DNA isolation, PCR to Sequencing?

I need a software to organize the reactions of PCR, where all the details of the experiment are available in one software from the date the samples arrived to lab, DNA isolation, PCR, Gel...

23 December 2015 2,988 2 View

Is anyone an expert on establishing a stable cell line expressing NMDARs?

Any help would be highly appreciated :)

13 December 2015 6,742 3 View

How can I calculate the cell voltage (V) for supercapacitor?

When calculating the energy density for supercapacitor cells we use the following equation .. I wish to know how to calculate the cell voltage (V) which is determined by the thermodynamic...

13 January 2015 239 6 View

Looking for an excel document/ software where you can compile qPCR data, normalize with housekeeping genes, generate graphs for comparative Cts etc.

I need one sheet/ program that compiles all the calculation together rather than doing each step separately.

01 June 2013 4,141 4 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

No title

02 March 2021 3,060 3 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View