Can anyone share the protocol to isolate RNA, DNA and protein using trizol?

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Soner Öner-Sieben

Hi,

the protocol from Thermo is quite useful. NOTE: Don't use Urea for dissolving protein, you WILL destroy your samples.

Best

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=&ved=2ahUKEwjl-uvjv6vyAhURZMAKHWIhDmsQFnoECAQQAQ&url=https%3A%2F%2Ftools.thermofisher.com%2Fcontent%2Fsfs%2Fmanuals%2Ftrizol_reagent.pdf&usg=AOvVaw0WftihiF82qcZY_JpQvDIY

Valeria De Arcangelis

Hi, in theory the answer is yes, pratically I don't recommend you to use the procedure for protein extraction, the yield is really poor

Valeria

Garima Jain

thank you Valeria De Arcangelis for recommendation...