Can anyone share the protocol for native PAGE using discontinuous tricine buffer system.? Intended to observe bacteriocin ingel activity?

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How the slurry is preparing for cathodes in batteries?

13 June 2024 4,314 3 View

Geology Doctorate. Available for Post-doc and guest faculty. Please give suggestions. Anywhere in world ?

Geology Doctorate. Available for Post-doc and guest faculty. Please give suggestions. Anywhere in world.

09 June 2024 1,805 4 View

How can analyses a split plot design by SPSS?

02 June 2024 5,946 2 View

What do you mean by meadow plant?

01 June 2024 7,248 2 View

What is the actual difference between Competency-Based Education (CBE) and Outcome-Based Education (OBE)?

In any professional education students' Competency is Evaluated and used as the measurement of Outcome of the teaching-learning process. Then how these two are differentiated in curriculum...

22 May 2024 8,488 25 View

Can I purify mononucleosome from T-cell using a kit followed by SEC superdex 200 10/300 column chromatography for CryoEM ?

I am planning to purify the mononucleosome for cryoEM study. I have seen, people assemble the nucleosome from recombinantly expressed histones and then providing DNA sequence to it. Isn't this...

19 May 2024 7,170 1 View

How can I prepare a loading dye that also act as a stopping buffer for an enzymatically degraded DNA, for analysis on a sequencing gel?

I have a recipe of loading dye (10 mM EDTA, 1X TBE, pinch of Xylene cynol and Orange G dye which is made upto 10 ml with 100% Formamide) but somehow the bands of the nucleotide product...

17 April 2024 8,025 6 View

How perform subpixel level measurement of deformation using image processing?

Hi, I am working on subpixel level measurement of deformation using FPGA with the help of image processing technique. Can anybody suggest me the steps to be followed? As per citation pixel level...

14 March 2024 4,078 0 View

What is the performance parameter for imblance dataset ?

I am curr research at phising detection Using URL . Using logistic Regression model . I have data set 1:10 ratio 20k legitimate and 2 k phishing .

10 March 2024 9,531 2 View

How much amount of zeolite 13x is required to mix with how much amount of water vapour to attain a temperature of 110 degree centigrade ?

I am trying to work with zeolite material instead of electrical heating element for drying, so how much amount of zeolite 13x is required to mix with how much amount of water vapour to attain a...

17 February 2024 3,177 0 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View