Can anyone please help me in processing SAR SLC data to generate interferogram using SNAP?

I have two scenes of the study area, one top and the other bottom. As per the tutorials and papers published, the step include coregistering the master sand slave images. However, when i try to...

24 February 2021 6,372 1 View

Regarding to presentation of pairwise comparison of data?

22 January 2021 8,648 1 View

How can we find crop transpiration (Tr) value from ETo values in Aquacrop model?

it like how we can get Tr value separately from ETo values in Aquacrop

20 December 2020 5,817 3 View

How to find load / force acting on the top face in ansys workbench?

The image attached is the representation of my application. This is an assembly of three items (top plate, bottom plate and the 5 load cells). The bottom plate is fixed and a load of 40 Kg is...

15 December 2020 1,145 6 View

Unlikely condition in the water inundation area after the cyclone?

I have done flood mapping with Sentinel 1 data for 3 districts of India (West Bengal) i.e. North Pargana, South pargana and East Medinipur. North and South Pargana are showing increasing trend in...

11 December 2020 4,714 3 View

How to calculate Area-Weighted Average Temperature at outlet in Ansys?

Suppose a fluid is flowing between two parallel plates (and exchanging heat) for 100-time steps. So, there is an area-weighted Average temperature at every 10 steps. How can I write a UDF to...

29 November 2020 6,240 2 View

Can you please recommend articles or book for in depth cocrystal information?

I am looking for information about coformer screenig, feasible method of synthesis at laboratory scale, cocrystal screening, solubility study, Dissolution study of powders, FT-IR analysis, DSC...

10 November 2020 413 6 View

How to know from DSC thermogram that cocrystals are formed or not?

I got three endotherms after doing DSC of physical mixture of Drug and coformer what this Indicate?

06 October 2020 350 4 View

What is apparent Km Value of an Enzyme, Is it different from usual Km value and Why it is important?

It is very much confusing when some papers use this term "apparent Km of an enzyme even if they use single substrate ? Not at all clear ! Please help me understanding this. Thanks in advance.

27 September 2020 3,522 7 View

What are the different revese crosslinking protocols?

I need to crosslink my cells for my experiment and at the end, I need to reverse crosslink and extract the proteins. Can anyone tell me what protocols are there for reverse crosslinking after...

23 July 2020 6,257 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View