Can anybody please tell me the detailed protocol for
1.studying microsporogeneis?
2. pollen -stigma interactions in interspecific hybrids using Fluorescent microscopy?
3. How to prepare the samples for studying pollen morphology using SEM and TEM?
4. How to prepare permanent slides for studying meiosis and mitosis in plants?
Waiting for your kind information...
I am in Banglore, if anybody is generous to teach me these techniques I will be very much thankful. We can have collaboration...
Samples
Dry, as follows: a. You took mature flowers from dry samples, placed in hot water
For the purpose of moisturizing for a period of between 20 - 30 minutes, after which the whole flower was transferred to a slice
Clean glass and placed a drop of water on it. The flower was then opened with two needles for two minutes to separate it
, The mature stalks were isolated and transferred to another glass slide placed on them by a drop of dye
Safranin and Glycerin. After the under the duck in the drop and moved to exit the pollen from them, then
The remaining part of the jacket was removed and the slide cover was removed and then ready for optical inspection.
The scanning electron microscopy can take your samples directly and open the door on
Clean glass slide and then spread the vaccine on it and then transfer it to the adhesive tape special and length of 1 cm and attached to another glass slide
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- Science method
- Similar topics
- Biological Science
- Ecology