Can anyone share a protocol for cryopreserving adipose tissue, in order to use it for confocal microscopy?

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Daniela Rodler

We had good success, when we wrapped a small piece of adipose tissue in aluminium foil and then immersed it in N2 cooled isopentane.

Best regards, Daniela

We had good success, when we wrapped a small piece of adipose tissue in aluminium foil and then immersed it in N2 cooled isopentane. Than you can soak the tissue in OCT and cryosection it in a cryostat at 10 micrometer.