Ioannis Drosos

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Questions related from Ioannis Drosos

Any help for setting gates when looking for lymphatic endothelial cells in adipose tissue?

I am trying to count the numbers/percentages of lymphatic endothelial cells in different mouse adipose tissue depots. These cells are theoretically CD31+CD45-Lyve-1+Podoplanin+, with the...

06 December 2018 4,272 0 View

Can anyone share a protocol for cryopreserving adipose tissue, in order to use it for confocal microscopy?

We need to cryopreserve small pieces of human adipose tissue (~250mg) in order to cut cryosections for confocal microscopy.

08 July 2018 840 2 View

How can I get nice peaks in a PI histogram for cell cycle analysis?

I am setting up a cell cycle analysis experiment using FACS. I stained cells with PI. I have tried both with adherent and non-adherent cells (A549 and THP-1 cells). Protocol seems to run fine. My...

14 October 2015 7,857 7 View

Can someone help with isolating primary human adipocytes?

We are trying to isolate adipocytes from human adipose tissue, using collagenase. We cut tissue into pieces and mince using a scalpel. After that we incubate tissue with 1-2 ml of 1.5mg/ml...

09 October 2015 3,098 7 View

How can I get rid of blood leukocytes when performing FACS in tissue single-cell suspensions?

I wish to perform FACS analysis of leukocyte populations in tissue-derived single-cell supsensions. Is there any way one can get rid of blood leukocytes that interfere in the analysis? I want to...

31 July 2015 5,254 3 View