Ioannis Drosos

3 Questions 6 Answers 0 Followers

Questions related from Ioannis Drosos

How can I get nice peaks in a PI histogram for cell cycle analysis?

I am setting up a cell cycle analysis experiment using FACS. I stained cells with PI. I have tried both with adherent and non-adherent cells (A549 and THP-1 cells). Protocol seems to run fine. My...

14 October 2015 7,841 7 View

Can someone help with isolating primary human adipocytes?

We are trying to isolate adipocytes from human adipose tissue, using collagenase. We cut tissue into pieces and mince using a scalpel. After that we incubate tissue with 1-2 ml of 1.5mg/ml...

09 October 2015 3,083 7 View

How can I get rid of blood leukocytes when performing FACS in tissue single-cell suspensions?

I wish to perform FACS analysis of leukocyte populations in tissue-derived single-cell supsensions. Is there any way one can get rid of blood leukocytes that interfere in the analysis? I want to...

31 July 2015 5,242 3 View