Can anyone send me the method for bacterial CFU count using SYBR Green?

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Karen A. Darbinyan Popular answer

Hello, see attached info.

Good luck!

Mert Sudagidan

Dear Dasharath, extract DNA from different concentrations of bacteria (CFU/ml or direct bacterial number) and plot a calibration curve after Real-Time PCR using SYBRGreen. In the attachment, there is a paper describing some primers.

Best regards.

Alireza Mordadi

With full respect, the method you are going to use is not proper at all.

We do CFU/ml to count the number of live cells but with your method you will count dead and live cells.

Regards.

Karen A. Darbinyan