SCREENING Can anyone say what the methods of "screening" and selecting transformant bacteria are and an example of each ?
I need to do immunohistochemistry in mouse brain, particularly in neurotensin-cre mouse line.
22 May 2024 3,565 7 View
citations
27 March 2024 5,206 1 View
I need information of methanol requirement for 1 MGD Waste Water Treatment
21 March 2024 8,516 3 View
How university student use and abuse the internet
11 March 2024 1,578 1 View
i WOULD LIKE TO UPDATE MY BIOGRAFICAL INFORMATION . For instance I am not anymore involved ad the Institution and department Ghent UniversityMUSEUM . i TRIED TO CORRECT THIS BUT WITHOUT SUCCES.
05 January 2024 1,350 2 View
i mean...i can't seem to find it in the Beall's list of potential predatory publishers and journals. The website interface of the ijrsi seem suspicious, and i've got email notified me that if i...
27 December 2023 3,081 5 View
In a well-built apartment building, each apartment has its own walls, and there is an air gap between the walls of adjacent apartments, which provides excellent sound isolation. Could a similar...
17 November 2023 3,174 9 View
Include the make and model.
14 October 2023 2,385 3 View
People have noticed centuries and millennia ago that ethnic groups and peoples differ from each other. I am looking for quotations from ancient times that contain judgments about the intelligence...
02 May 2023 7,964 4 View
I am developing a UHPLC method for content and purity for a peptide based on 100mM KPF6 and 100mM NH4PF6 pH 3.5. TFA as an ion pair reagent does not give the required separation performance. My...
20 April 2023 9,370 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I am conducting my analysis using SPSS. I log transformed my data using In(X+1) as my data contain zero values. However, when I want to back transform the regression coefficients generated from my...
31 July 2024 7,860 3 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View