Problem about anti-dopamine transporter immunohistochemistry. why the slides look strange and dark?

I perfused the mice, fixed the brains and used the Microslicer to cut to slides then used the avidin-biotin-peroxidase (ABC method) to do immunohistochemistry analysis. the method for details I...

17 November 2018 6,137 2 View

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05 September 2015 7,820 7 View

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30 August 2015 2,864 3 View

What is the confidence interval of a survival curve?

How to calculate the confidence interval of the survival curve based on Kaplan-Meier estimation? The general used formula doesn't seem to be the correct one. As the time goes on, the sample size...

14 April 2015 8,593 6 View

Why did my SH-SH5Y cells die after lentivirus infection even without any drug selection?

I tried to make a stable cell line using SH-SY5Y cells. The cells were infected twice with fresh virus. After the 2nd infection, the cells looked good. I changed the fresh media (10%FBS/DMEM) for...

10 April 2014 661 1 View

How can I get the spatial frequency component?

Recently, I saw a method that we can obtain a spatial frequency component of the stereoscopic images by applying the Sobel operation. But I don't understand how to get the spatial frequency...

13 November 2013 8,623 4 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

How to calculate battery consumption for IoT device?

Hi, everyone I'm working on a privacy-preserving algorithm that will apply to IoT devices. So, I need to measure the power consumption by implementing this algorithm. Any suggestion, advice,...

25 February 2021 8,749 6 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

How to troubleshoot Electrochromic Films and Electrochromic Devices?

I am trying to characterize an electrochromic system. The system uses ITO electrodes on glass, WO3 as the electrochromic film, and NiO as the ion storage layer. When I deposit NiO and WO3 and...

23 February 2021 9,592 2 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View