09 June 2014 3 8K Report

Good reads on phylogenetics.

More Sonja Blaauw's questions See All
What spores would these be?

Good day, Please could you help me identify these spores found in wheat roots? The foliar symptoms excipt that of Soil-Borne Wheat mosaic Virus hence I was looking for Polymyxa graminis but...

12 October 2023 2,937 1 View

Geothermobarometers for Amphibolite/Granulite?

I recieved EMP data from zoned amphibole, clinopyroxene and plagioclase from my rock sample. It doesn't contain garnet, only few biotite, some quartz and carbonate minerals. In my opinion...

08 February 2022 5,812 0 View

Is there a possibility to purchase human isolated mitochondrial DNA?

Hi everyone, I am currently trying to establish an assay based on the oxidation of isolated human mitochondrial DNA. I have been trying to isolate the mtDNA myself, and oxidize it afterwards,...

03 November 2021 5,937 0 View

Seahorse assay: when is the OCR too high so the O2 level (mmHg) gets too low (unintended hypoxia during the run)?

During Seahorse mitostress assays, we have great variance between different cell lines regarding their oxygen consumption. One cell line is tricky because at relevant confluency, the basal OCR is...

23 August 2021 3,017 2 View

What fungi or mold is this isolated on pda-C from a wheat leave?

Goo day; Wheat samples were bought into the lab with yellow stripes and mosaic-like symptoms on the leaves. The diseased leaves were sterilized with 1% NaOH and plated out on pda-C. plates were...

22 August 2021 1,810 9 View

CyQuant cell prolif. assay - how old can it be and still work??

Hi, I have frozen two 96-well plates with cells that I need to stain using the CyQuant Cell Proliferation Assay from ThermoFisher (C7026). However, I didn't realize that we only have some very old...

06 January 2021 448 4 View

What are these round structures found inside Wheat roots?

Good day, please assist me with the following. what are these Round structures found inside Wheat roots? Roots were in KOH Solution overnight and autoclaved for half an hour. Then viewed und a...

10 August 2020 9,039 3 View

Protein:Lipid analysis of pollen. Bradford vs. BCA Pierce?

I am interested in comparing the protein:lipid of pollen between three sister plant species. This information will then be linked to pollinator communities and rate of visitation to each of the...

16 May 2020 7,418 0 View

SPSS: How to obtain the CI for standardized bèta's?

I performed multiple linear regression, and for my report I need the 95% CI of the standardized bèta's. However, SPSS only gives the 95% CI for the unstandardized B. How to obtain the CI for...

28 April 2020 2,638 3 View

Subgroup analysis in small numbers allowed?

Hello, I had a question regarding subgroup analysis in small patient numbers. I am currently writing a paper on very rare tumours and the response to therapy in a real-world cohort, so no...

18 March 2020 5,225 16 View

Similar questions and discussions
What PCR barcoding primer can be used to determine a species in the Bursaphelenchus genus?

I am learning to barcode nematodes and was wondering which primer can be used to determine a species in the Bursaphelenchus genus. I do not need to maximize the phylogenetic relationship, just a...

01 February 2024 5,207 0 View

I have completed a phylogenetic network, but how can I view the character states of each node?

I'm a beginner with SplitsTree 4. I have completed a phylogenetic network, but how can I view the character states of each node?

09 January 2024 7,991 0 View

Input files for Phylonet -recomp, -riataHGT?

I would like to ask what type of input data do these commands require. I am a little bit lost in the Phylonet manual. Is it possible to construct phylogenetic network in Phylonet using data such...

14 November 2023 2,434 0 View

Finding and doing phylogenetic statistics on discrete variables?

Hello everyone, I hope everyone is doing well and is keeping safe. I was wondering if someone could please help me in that I want to use statistical analysis to measure whether different genera...

30 October 2023 7,035 0 View

Is it possible to concatenate mtCOI and nuclear genes?

I'm currently working on resolving a phylogenetic tree, and I'm wondering if it's possible to combine both mitochondrial and nuclear genes to achieve a more accurate result.

05 September 2023 1,323 7 View

Power analysis for phylogenetic comparative analysis?

Hi all, I did phylogenetic comparative analysis of genome size and environmental factors using more than 120 species. The function I used is from the package phylolm: mod

19 July 2023 8,835 0 View

Phylogenetics on TNT: how to establish more than one taxon as outgroup?

I've been battling with codes and it's no use. There must be a way to select a group of taxa as the outgroup, but I couldn't find how. I suppose that the alternative path is to make the whole...

22 June 2023 9,302 2 View

Why some species of the genus Cupressus have been changed to Hesperocyparis?

What is the explanation of this change which has affected the well-known Cupressus lusitanica (I assume some phylogenetic studies) Is this a definitive change and should we use this new scientific...

22 May 2023 4,120 1 View

Phylogenetic methods with a small number of species?

I am working on a dataset with 65 individuals that belong to 6 species. I am trying to use a linear model that corrects for phylogenetic relatedness amongst individuals, however I am concerned...

01 May 2023 6,755 5 View

To what extent LD based pruning can effect phylogenetic analysis?

SNP filtering remain as the essential step before performing any population genetic analysis. Can any expert share his/her experience on phylogenetic analysis with and without LD based SNP pruing...

26 March 2023 1,372 0 View

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