CyQuant cell prolif. assay - how old can it be and still work??

Hi, I have frozen two 96-well plates with cells that I need to stain using the CyQuant Cell Proliferation Assay from ThermoFisher (C7026). However, I didn't realize that we only have some very old...

06 January 2021 244 4 View

Subgroup analysis in small numbers allowed?

Hello, I had a question regarding subgroup analysis in small patient numbers. I am currently writing a paper on very rare tumours and the response to therapy in a real-world cohort, so no...

18 March 2020 4,983 16 View

Hot to test differences of mean(T test) on all variables in overlapping samples?

Good afternoon, I am working on my thesis on team work and I need to compare if teams differ when I adopt the rule 'at least 2 members of the team gave valid data' (19 teams in total), against...

14 July 2019 5,957 5 View

How do I Obtain Permission to administer the Servant Leadership Questionnaire?

I need to use the SLQ for my dissertation studies.

08 August 2018 1,864 3 View

Searching for articles on experimental power role manipulation in social psychology, can you recommend me some literature?

Hello everyone, Since I am interested in the concept of power and theories about it (soc.psy), I would like to find a specific experimental manipulation of it - and so far I am doing terrible! So,...

02 December 2017 6,806 3 View

Anti-FLAG-tag antibody for flow cytometry in mouse without major background signal issues?

Hi there, Can anybody recommend an antibody that works well to detect FLAG-tagged protein in mice with flow cytometry? I am considering to use FLAG tag for generating a transgenic mouse. HIS tag...

26 May 2016 3,404 4 View

Day care quality: Did anyone work with the MELQO - Measuring Early Learning Quality and Outcomes?

We heard that the official version of the MELQO is not yet ready to use. But we wondered whether maybe someone has a research version of it or some other experiences to share. We would like to...

27 April 2016 9,234 1 View

Antibody doesn't work for proteins of HEK lysate?

Hallo, I am doing some western blot experiments at the moment and have some problems. I transfected a construct in HEK293 cells and loaded a lysate to sds gel and blotted the proteins to...

13 April 2016 3,452 9 View

Has anyone used one of the gene trapping constructs form KOMP to generate floxed mice and actually gotten a positive LacZ staining of targeted cells?

Me and two of my colleagues from different labs at my institute used three different constructs targeting 3 different genes. All genes seem to get deleted fairly well but unfortunately no one of...

23 March 2015 8,975 1 View

Why do my infected cells detach in flatbeds and not one by one like they usually do?

Hello. I'm working with rotavirus infections of cells. Usually when I infect with RRV the cells die very fast (as soon as 24 h.p.i) and then they usually detach one by one and you can observe a...

17 March 2015 6,402 2 View

Does the phylogenetic tree making tool IQTREE use evolutionary placement algorithm?

For my master thesis I am conducting a phylogenetic research and for this I'm using tools like MEGA, IQTREE, FigTree, etc to make a phylogenetic tree. I wanted to know if IQTREE integrates the...

14 February 2021 1,541 2 View

Is there an alternative to PGLS that considers phylogenetic signal when calculating the estimated value?

I have been working on a study trying to estimate body mass in mammals using a skeletal proxy in R. My dataset has high phylogenetic signal (Pagel's Lambda = 0.9) and I am interested in removing...

26 January 2021 6,997 3 View

Question regarding investigation of the nature of bacteria: whether it can cause human diseases or not?

Hi all, Considering that one has the whole genome sequence (annotated) of a bacterium and wants to know if it has the potential to cause human diseases, what steps would you recommend...

26 January 2021 8,732 3 View

Are phylogenetic independent contrasts still used in biology?

I have a regression equation in which I am curious if the amount of scatter around the regression line is driven by phylogeny. I know there is a lot of phylogenetic signal because Pascal's lambda...

22 December 2020 937 1 View

EDGAR - How to add Whole genome sequence of the strain (novel) to EDGAR comparative genomic tool for comparison?

Hi all, I am interested to do a comparative genome analysis using EDGAR comparitive genomic tool. I have a novel bacteria whose genome sequence was obtained by us and now I was to do compare this...

10 November 2020 3,489 3 View

Which methods (or modern programms) are used to assess the biodiversity of highest vascular plants?

27 October 2020 240 3 View

R package for GLMM with phylogeny (but not for community composition)?

Hello, I have a dataset derived from a multi-species experiment that I carried out. I have 30 different plant species from several families and 3 geographical groups. I applied 6 different...

26 October 2020 874 3 View

How can I perform pairwise protein alignments using BLAST, but without search?

I have a set of about 1000 homologous protein sequences with high diversity across various genera and families. What I need is to perform BLAST alignment of each possible sequence pair and obtain...

25 October 2020 1,199 5 View

What are the genetic/physiological differences between deep water rice cultivars of North-Eastern India from that of South East Asian Countries?

Deepwater rice cultivars found in various regions around the world have their own differences. Please mention how the deepwater rice cultivars of North-Eastern India varies from that of Southeast...

23 October 2020 1,333 3 View

Has anybody used XLSTAT software for phylogenetics/Phylogeography?

Need a detailed tutorial on XLSTAT ( Input type format, output file, drawing conclusions etc) Unable to find online.

07 October 2020 6,764 4 View