Can anyone recommend me an universal primer pair for bacterial lipase gene amplification from environmental metagenomic samples?

RAJAL DEBNATH @Rajal-Debnath

05 December 2013 1 7K Report

I am interested in any program for degenerate primer designing other than CODEHOP.

Fabrice Armougom

You can try this tool :

http://bibiserv.techfak.uni-bielefeld.de/genefisher2/

or this one (but you have to download the software and used it locally:

http://acgt.cs.tau.ac.il/hyden/

Web_service here:

http://www.changbioscience.com/primo/primod.html

reccently 2013: in this article :

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3600133/

hope it can help you

Fabrice

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