Did anyone use Mag Flu4 to monitor intracellular Ca++ changes? Does that work?
The dye has a better affinity to bind Mg++ and people use this to track Mg++ changes. The company (LifeSciences) claims that the dye also binds Ca++ with lower efficiency. I'd like to know if it worths trying this dye to track Ca++ transients. We got it accidentally but I'd still use it if it works with Ca++. Does anyone have experience with it?
Hi Josh!
Thanks a lot! This is indeed a great news. This means that we may want to try to use it. We worried that due to the high Mg++ affinity we measure some ind of Mg++ signal instead of the desired Ca++ transient. But.. if other people used to image Ca++ concentration changes it should work for us a well.
Thanks again for the prompt response!
best,
Bela
Bela
Just tried Mag-Fluo 4 in the isolated mouse heart for Ca imaging with some interesting results. The dye was loaded at room temperature. Upon warming up to 37C, cytosolic Ca transients were detected very nicely. However, as the cytosolic signals decreased gradually, the sarcoplasmic reticulum (SR) Ca transients increased. After 30 min of warm up, all cytosolic Ca transients disappeared, which means, the dye is not good for cytosolic Ca measurement. But, I think it's potentially good for SR Ca imaging, considering the has an in vivo Kd of ~100 µM.
Thanks for the note. This is definitely something that we have to consider when using Mag-Fluo4.
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