Why do my cells die during transfection with JetPei?

Hello everyone, two months ago I did a transfection of HEKs cells to insert a miR using the JetPei reagent. On that occasion, the transfection was highly efficient and the cells were in perfect...

03 February 2021 1,296 6 View

Will the spread of the new SARS-Cov-2 strain affect the efficacy of mRNA-based vaccines?

Recent studies have highlighted the isolation of a new strain of SARS-Cov-2. Could the new discovery compromise the effectiveness of the vaccination campaign ???

21 December 2020 7,516 4 View

Why was sonication unsuccessful for 1 of 6 samples?

I have sonicated six HeLa-cell samples in RIPA lysis buffer (prior inc. on ice, 15 min). For one of these samples, the sonication was unsuccessful. My question is what factors should be taken into...

17 December 2020 8,531 2 View

Maskning for polysterene substrate?

We want to deposite a fine pattern ( 100-300 nm thick and 10 microns wide pattern) of Pt as temperature sensors on a polysterene substrate with a glass temperature of 95 degrees C. How would...

12 November 2020 9,761 3 View

Is temperature measurement really useful as a screening measure for COVID-19?

The measurement of the temperature at the entrance to some structures or hospitals is a universally adopted measure in prevention strategies. There are two questions: considering the high number...

31 August 2020 5,843 12 View

Someone recommend to use CFSE to stain parasites as Trypanosoma or Leishmania species to detect cells infection?

I have seen that some groups use CSFE to stain some parasite species before infect cells to detect percetage of infected cells. Could I use CSFE to stain Trypanosoma cruzi and measure infection in...

15 August 2020 1,898 2 View

After the shocking case of George Floyd, should the physical restraint techniques used in the police and psychiatric settings be recoded?

George Floyd's case shocked the world. Physical restraint is a method used by the police but also in the management of psychiatric patients who pose a danger to themselves or others. In your...

10 June 2020 1,331 9 View

Can the political orientation of a medical / scientific journal editor influence scientific choices?If so, it'is morally and ethically acceptable?

Donald Trump's statements and the political opinion that publishers, especially American publishers, had of him profoundly indicated the editorial lines of the main scientific newspapers. Most...

10 June 2020 8,913 15 View

Barriers and Factors with wound are discharge planning, how to narrow down this topic?

I'm DNP student that wants to discuss wound care discharge planning for my research. This is a very broad topic, and needing help to narrow the topic down. Any ideas on wounds that need more...

29 May 2020 8,870 1 View

Why are some plasmids (such as pKD46) temperature sensitive? What happens at higher temperatures to cause plasmid loss?

I am trying to figure out how temperature sensitive plasmids are made/isolated and what the biochemical/cellular mechanism is that causes them to be lost/lose function at higher temperatures.

20 March 2020 6,815 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View