We obtained OD of 400 samples from vaccinated fish and control groups.
How can we recognize immunity levels in examined fish?
I believe you need to follow the manufacturer's instruction, otherwise you should compute a cut off or threshold for your, self constructed, ELISA. I'm not sure you'd purchased the ELISA kits or constructed yourself. By the way you would need a quantitative ELISA kits rather than qualitative ones.
Dear Swati,
This study was carried out according to new ELISA design and our setting up in our Laboratory.
How to calculate cut-off point and distinguish level of Antibody production?
Thanks again
Regards
I understand that you are detecting specific antibodies. You can analyze your results in OD or you can determine the antibody titer. The last option is better.
If you work with unique dilution of the samples you can determine the cut-off value with the OD of samples of control group (median +/- 2 or 3 SD).
You need to perform serial dilution of your samples if you want to determinate titers them.
In both cases you need to analyze your datas with a satistical test in order to probe if the differences between groups are significant or not.
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