Thought I'd ask the experts before buying. Thanks in advance!
In case anyone is watching for an answer, I found one that works well for IPs from Protein Tech Group, and it was super cheap!
Hi! I am looking at cortical neurons in coronal sections from mice expressing Thy-YFP and a top secret genetic manipulation :) I noticed these vacuoles in the apical dendrites of most of the...
01 February 2019 7,141 7 View
Hi! Some of the lentiviral transfer plasmids I'm using have this short form of the CMV promoter that is 350bp instead of 589bp for the usual CMV promoter. Does this short form have a name?...
10 November 2018 5,176 2 View
Hey Gang, Very easy question to someone who knows more about identifying glia than I do! I am using antibodies against S100 to mark astrocytes and Iba1 to mark microglia in a culture of primary...
05 June 2017 766 2 View
Hello ResearchGate Community! I'm building polycistronic lentiviral vectors for the expression of multiple fluorescently-tagged proteins using Multisite Gateway, and I'm finding that I get mosaic...
10 November 2016 6,116 2 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
03 August 2024 3,371 3 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
01 August 2024 7,199 1 View