What cut-off should we use when applying BUSCO to Escherichia coli (Bacteria) genome assemblies?

We would like to analyse thousands of E. coli genomes and identify orthologous genes. Before this we would like to filter the bad quality genomes by BUSCO (https://busco.ezlab.org/). What % of...

15 November 2020 5,152 3 View

Can you provide RT-PCR raw data to train our machine learning algorithm?

Dear colleagues working with RT-PCR devices, We are a team of data scientists, biologists, web developers working on an open-source machine-learning algorithm to contribute suppressing the COVID19...

06 May 2020 1,789 3 View

How to calculate R2 in FIRTH LOGISTIC REGRESSION?

Dear all, I have a very big sample with very few positive cases - so I decided to run a Firth Logistic Regression in SPSS. However, the program does not provide the squared R. Do you know a...

18 November 2019 4,542 3 View

Is there a procedure that I can use to remove the N-terminal acetylation on a peptide and increase the solubility?

I am working with a series of 13-mer peptides that are quite hydrophobic. Most of the peptides do not contain any acidic or basic residues. The peptides are capped at the ends (N-terminal...

06 July 2016 6,756 3 View

Hi! Question about Hierarchical regression to test interactions.

Hi! I used Hierarchical regression to test interactions - step 1: main effects step 2: three 2 way interactions step 3 one 3-way interaction The R-square difference between step 2 and step 1 was...

11 January 2016 9,589 8 View

Is this true that 6M GdnHCl will degrade my peptide if I try to purify the peptide using 6M GdnHCl to dissolve it?

The peptide does not contain any acidic or basic residues and it is capped on the ends. We tried to dissolve the peptide in several organic solvents. It dissolved in some of the solvents, but...

25 June 2015 9,665 3 View

What is the most important thing in your life?

maybe science, family, learning?

01 January 1970 3,812 82 View

Importance of ethics in education?

What is your opinion on the issue of ethics? Do you emphasize its importance to your students, or you believe they had a chance to get familiar with ethics at previous modes of study(primary and...

01 January 1970 633 11 View

Is it better to attend a conference or just participate?

I also like to perform.

01 January 1970 3,416 19 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

No title

03 March 2021 5,360 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View