Can anyone provide the vector map of pAM-PAT 35S YFP vector?

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Is it possible to use the Fused Deposition Modeling (FDM) to additively manufacture interconnected porous structure generation of >100-200 micrometer?

Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...

09 August 2024 7,892 0 View

How can we deal immediately with a person having anxiety attacks?

there can be some triggering situations, how do you handle them?

18 July 2024 7,076 3 View

What is the degree of crystallinity of a crosslinked polyethylene (PEX-A) pipe? Is there some kind of index? If yes, where can I find them?

I need the crystallinity percentage value of crosslinked polyethylene (PEX-A). I know that they have more than 70% crosslinking. Is there any way to calculate the crystallinity percentage from...

22 April 2024 3,007 0 View

How to check dipole-dipole interaction strength between two molecules when H bonding may not be the stabilising factor in a dimer molecule?

I have optimised the geometry of the dimer of a molecule using pcm solvent model and B3LYP functional with 6311++G(d,p) basis set in Gaussian 09 software. There is possibility of C-H...O H bonding...

01 April 2024 1,820 1 View

Collaboration Wanted: AI in Manufacturing (Cyber-Physical Systems/Digital Twins) for Journal Publication?

Hello, I'm seeking collaborators interested in researching and developing AI applications for Cyber-Physical Systems (CPS) or Digital Twins in the manufacturing sector. My goal is to publish a...

17 March 2024 1,116 3 View

How to dissolve Pyrazinamide to administer in mice?

Hi, I intend to give mice 150 mg/kg of pyrazinamide. I need 3.7mg per mice and 100ul needs to be administered. The drug only dissolves in DMSO and the final amount of the DMSO in water exceeds...

27 September 2023 6,416 0 View

Without measuring lifetime, how to distinguish between fluorescence and delayed fluorescence?

The PL int will be higher in the case of Fl, but both are supposed to overlap. Then how do know if the system has delayed Fl emission?

05 September 2023 3,068 4 View

How can I calculate the empirical orthogonal function?

I want to calculate empirical orthogonal functions using Python. but I don't know the method how I can calculate EOFs. Please guide me about it and if anyone has a code for calculating it, please...

27 July 2023 8,703 0 View

How a flame within deep tube well water in remote village can be explained as well as eliminated ?

Image Ref : The Telegraph Online ( 16.06.18 )/ 19.07.2023.

18 July 2023 397 8 View

Can we re-incubate the western blot membrane for the same protein with same primary and secondary antibody?

Hi. I'm trying to extract IFN-G from transfected CHO cells. I have performed western blots a few times with both transfected and non-transfected CHO cell lysate samples, and I'm not getting...

27 May 2023 1,127 3 View

Geotechnical Engineering (Proceedings of the ICE) time review?

Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...

10 August 2024 6,493 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?

31 July 2024 8,939 5 View

What should a Mechanical Engineering PhD scholar focus on during their PhD to enhance their chances of securing a postdoctoral position?

29 July 2024 7,714 4 View

May I know the exact Quartile of the journal- Advanced Engineering Materials (Wiley) for material science category?

In some data sources it has been grouped in Q1 and some shows it is Q2.

29 July 2024 4,227 2 View

Ternary in Electrical Engineering?

Dear Research Community I would like to invite Elctrical Engineering specialists to solve problems related to Ternary Algebra or tripple sets I have an algorithm that places in relation three...

28 July 2024 5,771 2 View