I am interested in: CMV-GFP, CaMV-GFP, Verticillium dahliae-GFP and Pyrenochaeta lycopersici-GFP.
Hi to everyone! Does any of you have experience in transfecting siRNA in Jurkat cells and can provide a reliable method? I have both Neon transfection system and RNAiMAX Lipo, but to date none of...
30 June 2024 6,901 4 View
I'm doing my first research and I can't find a way to see the shoreline evolution.
09 May 2024 1,817 4 View
I run an experiment to test the dispersing property of oxalic acid on TiO2 (anatase) colloids, only by mixting the carboxylic aqueous solution with the titania colloid. My results showed that a...
08 May 2024 4,667 0 View
Hi, I would like to set up an experiment with some plants sprayed with an aqueous solution of 200 μM Methyl Jasmonate with 0.025% Zipper surfactant. From the Sigma/Merch website I see I can buy...
05 February 2024 8,534 1 View
For my research, I need visual and/or auditory event related potential, EEG data from normal patients and patients with Alzheimer's disease. Can anyone suggest where I can find them?
29 September 2023 3,831 3 View
In the liquid phase sintering of Tungsten-Iron-Nickel powder compacts, could the presence of carbon (between 0.6% and 1.5%) significantly influence the final microstructure by altering the...
30 July 2023 4,368 1 View
Good morning, everyone! We are doing a spatial analysis to identify the best sites to install centralised upgrading plants. These plants should collect the biogas produced in several anaerobic...
28 June 2023 1,811 2 View
Hello everybody, I'm a master degree student. I'm working with 16S data on some environmental samples. After all the cleaning, denoising ecc... now I have an object that stores my sequences,...
25 May 2023 6,380 2 View
I measured by DLS the size of a TiO2 nanoparticles suspension sample to compare the results against a TEM measurement (5-6nm). As I expected I got different results from the two experiments due to...
24 May 2023 7,592 8 View
I want to use IEEE 14 bus system to integrate two microgrids in the generator buses. My research would be based on Model predictive control to schedule charge and discharge on on BESS of the...
26 March 2023 1,574 3 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?
31 July 2024 5,087 4 View
What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?
31 July 2024 7,921 5 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
How microorganisms are important for maintaining of healthy soil and biodiversity and microorganisms and plant roots contribute to soil formation?
31 July 2024 8,939 5 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...
28 July 2024 5,658 0 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View