How to regenerate PES Membrane of centrifugal filtration tubes having 5 and 50kDa MWCO?

Dear Collegues, I am using a relevant number of centrifugal filtration tubes made of polyethersulfone (PES) with 2 different MWCO, 5 and 50kDa for separation of protein in milk. They are quite...

15 December 2020 7,951 3 View

How to sterilise maize seeds in the lab?

What is the most effective protocol to sterilise maize seeds in the lab? We have tried few common procedures but with no luck (still get fungus contamination on agar plates): 1)10% sodium...

19 November 2020 602 15 View

How I can simulate solid state sintering?

I want to simulate solid state sintering of metal powder, conducted under vacuum. I am interested in the initial stage of sintering, does not matter to reach full density. Do you know any model or...

24 September 2020 7,383 5 View

Is there a good library to perform Petri net simulation in python?

Hello, I have designed several petri nets for my research. I am wondering how can I simulate them and gather system performance measure (eg sojourn times, throughput) using python? Is there a...

11 May 2020 2,120 2 View

What are the relevant economic, technical and environmental aspects of the recycling of batteries for automotive applicantions?

A paper review. Could you please provide some researches about the topic?

28 April 2020 946 5 View

Difference between photovoltaic plant short-circuit current and synchronus generator short-circuit current in AC transmission system?

Hello, I understand that photovoltaic plants are able to disconnect very quickly if there is a fault in the grid (cause power electronics), while synchronus generators cant disconnect due to his...

30 October 2019 8,457 5 View

Psychic salience has been studied in his correlation with psychosis.What about psychotropic drugs effect?

Some drugs seems to raise the feeling of psychic salience while others dont.

20 September 2018 3,556 1 View

In psychotic patients, psychotic features of the speech can vary their gravity during an interview. What do you think can be the influential factors?

In my view, these factors can be: the emotional meaning of the theme for the patient, anxious sympyoms caused by the situation or the interviewer, a relatedness feeling with the interviewer, a...

03 September 2018 303 3 View

Which assay to detect human serum levels of anti-troponin-I, anti-troponin-T and anti-tropomyosin antibodies?

We are currently using indirect ELISA to measure human serum levels of anti-troponin-I, anti-troponin-T and anti-tropomyosin antibodies? This indirect ELISA is not sensitive enough for our...

23 May 2018 6,546 2 View

Ksp in aprotic polar solvent , any idea?

11 May 2018 2,772 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View