I need the details of MFT which is used for Mycobacterium tuberculosis assay.
Hi, I am preparing organic solvent extracts using cell free supernatent or spent medium using separating funnel. For the same, I took spent medium and organic solvent in 1:1 proportion but somehow...
05 June 2016 6,589 13 View
Hi, I have started with organic solvent extraction of spent medium or cell free supernatant with butanol, but an oil globule-like substance is being formed in organic layer. I want to know how to...
02 March 2015 652 5 View
HI, I am trying to isolate the secondary metabolites of some marine bacteria using organic solvent extraction. I am going to use rotary vacuum evaporator in order to obtain the powdered form of...
01 February 2015 10,033 13 View
I am about to do organic solvent extraction but I am confused which one to use. The one with a temperature range of 40-60, 60-80 or 80-100.
03 April 2014 5,339 9 View
Hi guys, I am planing to work on a marine bioactive compound. I came across a bioactivity guided protocol. Now I am confused about the fraction to be used for bioactivity. Which fraction...
02 March 2014 7,464 3 View
I carried out glycerol stock preparation of bacterial culture using 20% glycerol. I left it for night in a refrigerator. When I checked net morning, the suspension inside the vial was found to be...
02 March 2014 10,033 33 View
I am planing to work on the yield optimization of natural products. While doing a literature survey, I came across the Placket and Burman Plot and Response Surface Methodology. Now I am damn...
02 March 2014 976 7 View
I am interested in anti-tubercular and want to know about a group working on it.
02 March 2014 6,384 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...
10 July 2024 6,830 1 View
Hi, I would appreciate professional and technical insights regarding primary cell culturing. I've been working on optimizing my primary cell culture, and while it is successful at times, there are...
10 July 2024 4,918 2 View