How to separate acetone:water, ethanol:water and methanol:water solution?

Hi, I am preparing organic solvent extracts using cell free supernatent or spent medium using separating funnel. For the same, I took spent medium and organic solvent in 1:1 proportion but somehow...

05 June 2016 6,505 13 View

How do you get rid of an oil globule-like substance forming during organic solvent extraction?

02 March 2015 596 5 View

What is the significance of DMSO in solvent extraction?

HI, I am trying to isolate the secondary metabolites of some marine bacteria using organic solvent extraction. I am going to use rotary vacuum evaporator in order to obtain the powdered form of...

01 February 2015 9,968 13 View

What is the ideal temperature for organic solvent extraction as per the petroleum ether is concerned?

I am about to do organic solvent extraction but I am confused which one to use. The one with a temperature range of 40-60, 60-80 or 80-100.

03 April 2014 5,277 9 View

What is the ideal glycerol concentration for glycerol stock of bacterial culture?

I carried out glycerol stock preparation of bacterial culture using 20% glycerol. I left it for night in a refrigerator. When I checked net morning, the suspension inside the vial was found to be...

02 March 2014 9,969 33 View

Can anyone provide me with the mouse foot pad technique of a tuberculosis assay?

I need the details of MFT which is used for Mycobacterium tuberculosis assay.

02 March 2014 5,361 0 View

What is the bioactivity guided fractionation of natural products and does it apply to bacterial products?

Hi guys, I am planing to work on a marine bioactive compound. I came across a bioactivity guided protocol. Now I am confused about the fraction to be used for bioactivity. Which fraction...

02 March 2014 7,404 3 View

What is the Placket and Burman Plot and Response Surface Methodology?

I am planing to work on the yield optimization of natural products. While doing a literature survey, I came across the Placket and Burman Plot and Response Surface Methodology. Now I am damn...

02 March 2014 911 7 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Hi everyone, I want to lower the pH of microbiological broth, what are the best options?

I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.

03 February 2021 6,959 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View