Can anybody refer me to a group working on anti-tubercular activity?

More Sandesh S Mandavkar's questions See All

How to separate acetone:water, ethanol:water and methanol:water solution?

Hi, I am preparing organic solvent extracts using cell free supernatent or spent medium using separating funnel. For the same, I took spent medium and organic solvent in 1:1 proportion but somehow...

05 June 2016 6,589 13 View

How do you get rid of an oil globule-like substance forming during organic solvent extraction?

Hi, I have started with organic solvent extraction of spent medium or cell free supernatant with butanol, but an oil globule-like substance is being formed in organic layer. I want to know how to...

02 March 2015 652 5 View

What is the significance of DMSO in solvent extraction?

HI, I am trying to isolate the secondary metabolites of some marine bacteria using organic solvent extraction. I am going to use rotary vacuum evaporator in order to obtain the powdered form of...

01 February 2015 10,033 13 View

What is the ideal temperature for organic solvent extraction as per the petroleum ether is concerned?

I am about to do organic solvent extraction but I am confused which one to use. The one with a temperature range of 40-60, 60-80 or 80-100.

03 April 2014 5,339 9 View

Can anyone provide me with the mouse foot pad technique of a tuberculosis assay?

I need the details of MFT which is used for Mycobacterium tuberculosis assay.

02 March 2014 5,428 0 View

What is the bioactivity guided fractionation of natural products and does it apply to bacterial products?

Hi guys, I am planing to work on a marine bioactive compound. I came across a bioactivity guided protocol. Now I am confused about the fraction to be used for bioactivity. Which fraction...

02 March 2014 7,464 3 View

What is the ideal glycerol concentration for glycerol stock of bacterial culture?

I carried out glycerol stock preparation of bacterial culture using 20% glycerol. I left it for night in a refrigerator. When I checked net morning, the suspension inside the vial was found to be...

02 March 2014 10,033 33 View

What is the Placket and Burman Plot and Response Surface Methodology?

I am planing to work on the yield optimization of natural products. While doing a literature survey, I came across the Placket and Burman Plot and Response Surface Methodology. Now I am damn...

02 March 2014 976 7 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,898 0 View

Isolation of Hydrogen Producing Bacteria?

any good research which can show selective microbial strains for producing hydrogen? Yes the normal procedure is known but the isolation is the key for such selective microbial strains which can...

13 July 2024 2,255 3 View

Hi. Can I get the list of publishers for microbiology-related short communications without publication fee (APC)?

I need to know which journals are exempt from publication fees (APC) in the area of microbiology

11 July 2024 6,346 2 View

Can I do IPTG induction on OD600=2.6 in TB?

I have been looking at papers for protein expression and trying to follow the process. I'm using TB medium to do the process, and the paper says to grow to an OD600 = 1.8 at 37 degrees, then let...

30 June 2024 6,502 5 View

What are the regulatory guidelines to submit a microorganism as Generally Regarded as Safe ?

Give in detail with available literature and website links for submissions

27 June 2024 9,753 1 View

What are these aggregates in activated sludge flocs?

I've been working with activated sludge samples and have encountered a challenge in identifying certain structures/organisms that appear abundantly within the flocs during optical microscopy...

23 June 2024 9,584 1 View