Sandesh S Mandavkar

9 Questions 14 Answers 0 Followers

Questions related from Sandesh S Mandavkar

How to separate acetone:water, ethanol:water and methanol:water solution?

Hi, I am preparing organic solvent extracts using cell free supernatent or spent medium using separating funnel. For the same, I took spent medium and organic solvent in 1:1 proportion but somehow...

06 June 2016 6,547 13 View

How do you get rid of an oil globule-like substance forming during organic solvent extraction?

Hi, I have started with organic solvent extraction of spent medium or cell free supernatant with butanol, but an oil globule-like substance is being formed in organic layer. I want to know how to...

03 March 2015 630 5 View

What is the significance of DMSO in solvent extraction?

HI, I am trying to isolate the secondary metabolites of some marine bacteria using organic solvent extraction. I am going to use rotary vacuum evaporator in order to obtain the powdered form of...

02 February 2015 10,003 13 View

What is the ideal temperature for organic solvent extraction as per the petroleum ether is concerned?

I am about to do organic solvent extraction but I am confused which one to use. The one with a temperature range of 40-60, 60-80 or 80-100.

04 April 2014 5,314 9 View

Can anyone provide me with the mouse foot pad technique of a tuberculosis assay?

I need the details of MFT which is used for Mycobacterium tuberculosis assay.

03 March 2014 5,404 0 View

What is the bioactivity guided fractionation of natural products and does it apply to bacterial products?

Hi guys, I am planing to work on a marine bioactive compound. I came across a bioactivity guided protocol. Now I am confused about the fraction to be used for bioactivity. Which fraction...

03 March 2014 7,438 3 View

What is the ideal glycerol concentration for glycerol stock of bacterial culture?

I carried out glycerol stock preparation of bacterial culture using 20% glycerol. I left it for night in a refrigerator. When I checked net morning, the suspension inside the vial was found to be...

03 March 2014 10,007 33 View

Can anybody refer me to a group working on anti-tubercular activity?

I am interested in anti-tubercular and want to know about a group working on it.

03 March 2014 6,360 2 View

What is the Placket and Burman Plot and Response Surface Methodology?

I am planing to work on the yield optimization of natural products. While doing a literature survey, I came across the Placket and Burman Plot and Response Surface Methodology. Now I am damn...

03 March 2014 953 7 View