Can anyone provide me protocol for Ultra Competent cells Preparation?

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Dachuri Vinay Kumar

please find the details in the attached document

Pranay A. M.

Dear Agni

Our lab prepares competent cells in CCMB 80 buffer and stores at -80C in 50UL aliquots. We have been using these for more than 6 months and were able to get good results. The protocol is available online.

Good luck!