I am studying RET/PTC rearrangement in thyroid cancer. But I do not have a positive control to validate my results. Can anybody provide me the positive control or suggest how I should proceed .
RET/PTC was once considered an exclusive hallmark of the papillary thyroid carcinoma and later it was also found sporadically in benign thyroid lesions.
There are two cell lines which are positive for fusinon oncogene RET/PTC.
FB-2 and TPC-1 cell lines, which derive from a papillary carcinoma, carry an activated RET oncogene with the RET/PTC1 rearrangement. RET/PTC rearrangements are chimeric transforming genes generated by the fusion of the RET tyrosine kinase domain to different heterologous genes.
FB-2
TPC-1
See the attached result of PCR amplification with primers specific for the RET/PTC1 rearrangement
Ref. DOI: 10.1002/ijc.10116
Thank you so much for the help.. I think thats how I will proceed. Will keep u updated.
If In my previous study I also detected the prevalence of RET/PTC1 and 3. TPC-1 was used as positive control for RET/PTC1 to view on gel electrophoresis. We have no positive control for RET/PTC3. The best way is to send for Sanger sequence.
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