Can I use Fluorescein Diacetate (FAD) to check the viability of my plant cells after the H2O2 treatment?

Hi all, I am treating my 8 day old Arabidopsis thaliana saplings with different concentrations of H2O2 for variable time periods. After the treatment I want to check the viability of cells...

10 November 2019 2,346 2 View

Is it required to quantify cDNA concentration before going for semi-quantitative PCR of any particular gene?

To prepare cDNA, I took the equal concentrations of RNA of both control and experiment samples. The volume of control and experiment samples during cDNA preparation was kept equal. Do I need to...

02 March 2017 9,756 6 View

How long overhang I can keep in my primer?

31 December 2016 153 7 View

Do I need to go for Dpn1 treatment?

07 August 2016 409 4 View

Why I'm getting extra protein bands with my specific protein band during Ni-NTA His-tagged protein purification?

I have expressed a protein of C. jejuni in E.coli M15 cells using pQE30 vector. My protein carries a N-terminal hexa his tag. I have purified my recombinant protein using Ni-NTA columns and I have...

07 August 2016 9,231 8 View

Transformation of E.coli, T7 expression strain with an expression vector having T5 promoter will work??

I have an expression vector carrying my gene of interest with T5 promoter and I want to transform it into BL21DE3 strain which is a T7 expression system. Just I want to know if doing so will not...

05 June 2016 2,074 3 View

How can I grow two different cell lines in one plate and even they don't merge in each other?

11 December 2015 2,725 4 View

What is the accuracy of epitope prediction software's?

I have no idea how these epitope prediction software's do work but I think using them will be somehow beneficial rather than designing primers blindly for the peptide synthesis. Before I use any...

08 September 2015 4,037 1 View

Why does my plasmid get stuck in the agarose gel well?

Hi, I've cloned my GOI into TA cloning vector( pDrive) vector, after I did transformation into DH5 alpha I got good number of colonies.After this when I'm doing plasmid isolation using...

07 August 2015 4,458 7 View

Can someone please suggest me the name of any tool or program where I can match two sequences side by side?

I want to know the name of any tool or program which I can download easily.I want to align two sequences side by side and and want to see the base pairs which are matching and the gaps. Also it...

04 May 2015 9,332 3 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View