Can anyone provide me a protocol for the isolation of nuclei from Saccharomyces cerevisiae.

More Somashekara S. Chaluvegowda's questions See All

Which is the more reliable bioinformatic tool to predict protein sumoylation?

Could anybody suggest me a bioinformatic tool to predict protein sumoylation.

21 August 2017 6,627 3 View

How to arrest Saccharomyces cereviciae cells in different phases of meiosis?

I need to arrest Saccharomyces cerevisiae cells in different phases of Meiosis. Can anyone provide me a protocol for the same.

04 November 2016 9,075 2 View

What could be the reason for some areas to be left as non sputtered?

I performed FE-SEM on hollow fibers and observed that some areas are not sputtered. Au/Pd alloy was used for sputtering. What could be the possible reason for such a scenario. Regards, Koushik...

08 October 2016 5,384 11 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...

01 August 2024 9,227 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Shin-Ichiro Hiraga

Here's a method paper by my colleagues, which includes nuclei isolation.

I hope this helps.

https://www.ncbi.nlm.nih.gov/pubmed/22465796

Sreelekshmi Mony

You can refer to the link below for the protocol

http://www.biolchem.ucla.edu/labs/grunstein/Yeast%20Nuclei%20Isolation.html

Somashekara S. Chaluvegowda

Thank you Shin-Ichiro Hiraga and Sreelekshmi Mony. I will try the protocols which you have suggested.