Can anyone provide me a protocol for the isolation of nuclei from Saccharomyces cerevisiae.

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Shin-Ichiro Hiraga

Here's a method paper by my colleagues, which includes nuclei isolation.

I hope this helps.

https://www.ncbi.nlm.nih.gov/pubmed/22465796

Sreelekshmi Mony

You can refer to the link below for the protocol

http://www.biolchem.ucla.edu/labs/grunstein/Yeast%20Nuclei%20Isolation.html

Somashekara S. Chaluvegowda

Thank you Shin-Ichiro Hiraga and Sreelekshmi Mony. I will try the protocols which you have suggested.