Hi there,
Does anyone have a VGLUT2-GFP mouse with good cell body expression in the spinal cord? I'm finishing up an anatomical and electrophysiological study for a subset of interneurons in the cervical spinal cord, and need a good marker for excitatory cells.
Any help would be much appreciated (and obviously acknowledged in the author block of the paper to be submitted at the end of the year). I'm able to travel to any lab in the world to perform the 2-3 week experiment, and can bring all my own equipment.
Thanks for your time.
Jamie
I just read your question, did you find a solution ?
I have living VGlut2-eGFP mice (Tg(Slc17a6-EGFP)FY115Gsat #11835-UCD, used by Li et al. 2013 doi: 10.1523/JNEUROSCI.3667-12.2013). Expression in the spinal cord is great in young animals, slightly faintest in adults. You can probably get them from the MMRRC with a better sanitary status.
Boris
there is this one too:
A transgenic mouse line for molecular genetic analysis of excitatory glutamatergic neurons.
Borgius L, Restrepo CE, Leao RN, Saleh N, Kiehn O.
Mol Cell Neurosci. 2010 Nov;45(3):245-57.
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