Can anyone provide a protocol for measurement of ROS in bacterial cells by methods other than flow cytometry?

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For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Does the mol. wt. of a tackifying resin affect on tackiness of a Adhesive ? If yes how ?

Effect of tackifying resin mol. wt. on tackiness of a Adhesive

21 June 2024 4,596 1 View

Difference between Radial and Linear SEBS ?

Difference between Radial and Linear SEBS, in view of processing and properties.

21 June 2024 2,927 0 View

Measuring fluorescence intensity in ImageJ?

Hello, I have a question regarding fluorescence intensity measurement in imageJ software. The tutorials provide different methods, the simplest one is to use a fixed ROI and measure the mean gray...

20 February 2024 752 0 View

How can polystyrene beads be added as a suspension in culture media?

I am trying to dissolve the PS beads in MS media as a treatment to plants. I can not make a suspension. I also require to measure Zeta potential of the PS beads, but the DLS analysis needs a...

04 February 2024 5,098 11 View

Elispot vs cultured Elispot?

Hi , My over all experimental goal is to define Zika virus-specific T cell responses in macaques infected with Zika virus. We also have control macaques that were not infected with Zika. We used...

16 November 2023 5,546 0 View

Why are there multiple bands beneath band of interest on SDS-PAGE gel after performing size exclusion chromatography?

I have been purifying proteins which have an N-terminal His-tag using Ni-NTA affinity chromatography. I carried out size exclusion chromatography using Superdex HiLoad, Superdex, and Superose...

24 September 2023 6,454 8 View

What is the treatment of choice for shoulder pain ?

It is very evident so far that although there are multiple conservative treatment for shoulder pain starting from medication to intraarticular injections to physiotherapy or naturopathy but none...

14 September 2023 6,588 7 View

What is the effect of di-block content (sis) on tackiness of a holt-melt adhesive?

What is the effect of di-block content (sis) on tackiness of a holt-melt adhesive....effect of viscosity and mol. wt. on tackiness & rheology

12 August 2023 6,351 0 View

Approximately how much time does it take for UV radiation to cause mutation in DNA?

Can higher exposure to UV radiation of my gel which I need to cut and purify create mutations for further use?

06 August 2023 7,620 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View