I have a protein whose probable function is RNAse. I have confirmed this by incubating the RNA with protein and loading the mixture on RNA gel. What I require now is a quantitative method to measure the activity parameters. As I have gone through the literature, I got a protocol based on spectrophotometric methods from a very old paper, maybe 1960s. Can anyone suggest any simple and easy methods to assay the activity?
Hi Rakesh, maybe have a look at this (the first one is the paper you mentioned?)
http://www.ncbi.nlm.nih.gov/pubmed/13911281
http://www.ncbi.nlm.nih.gov/pubmed/21001153
http://www.ncbi.nlm.nih.gov/pubmed/6663003
There are also kits available, which use Quencher/Fluorophore labeled substrates to quantify activity, e.g.
http://www.invitrogen.com/site/us/en/home/References/Ambion-Tech-Support/nuclease-enzymes/tech-notes/measuring-rnase-activity-.html
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