How can I increase cDNA concentartion obtained from viral RNA?

I need good quality and quantity of viral cDNA for NGS analysis. Please suggest how can I increase amount of cDNA obtained from viral RNA.

02 February 2021 8,379 3 View

Is there any low-cost filtering system (or other tech.)to lower the salt conc. (salinity) of surface/groundwater for large scale agricultural system??

The available water (surface and groundwater) is highly saline (6-20 dS/m). It is required to produce crop (moderately saline tolerant, up to 6 dS/m). Is there any low-cost filtering system (or...

18 January 2021 5,015 3 View

What will be the life of siRNA (liquid form) at -20 degree, if kept unopened?

Hi all, I just want to know that, if we keep the siRNA in a -20 degree freezer (as mentioned in the product detail), what will be the average life of the siRNA? Can we store for more than one...

10 January 2021 6,346 6 View

Can we compare regression results (models) for different groups?

Dear Research Gate Members, I have a query regarding comparing the two groups on the basis of the regression models For Instance: I have two sector companies i.e. Public Sector and Private...

09 January 2021 9,645 8 View

Can I add or delete studies from systematic review during revision stage of paper publication?

I have conducted a systematic review and one of the reviewers had mentioned about missing out on some good studies from the sample. on the surface the study is on a different topic but after an...

21 December 2020 573 4 View

Is it possible to grow human breast cancer cell lines in immune competent mice?

Hello everyone, I am working in breast cancer and wanted to do in vivo studies in breast cancer mice models. Here we do not have the facility to maintain immune-deficient mice, and I have MCF-7,...

27 November 2020 1,638 3 View

Pcambia 1301 sequencing ?

I got a LB plate of pcambia 1301 binary plasmid from an institute. I performed miniprep to isolate the plasmid. Now I want to sequence this plasmid. What kind of primers and reagents will be...

03 November 2020 6,075 4 View

Can I use gene specific primers to sequence my insert after TA clonning in pcr 2.1 ?

After ligating the insert in TA vector pcr 2.1, transformation and miniprep. I amplify the gene with simple gene specific primers . Now I want to sequence the insert . Which primers would I need...

31 October 2020 7,077 4 View

Can anyone suggest some potential target proteins for anticancer docking studies?

The plant extract, I have been working on, has a potent anti-tumor effect proven by several in vitro and in vivo studies. Through, the direct effect of the extract on tumor cells is not explained...

11 October 2020 2,935 4 View

What can I refer for Depth estimation using multiple camera input?

Currently, I am working on my final year project which involves depth estimation form multiple camera inputs for video analysis purposes. I am new in this field and struggling to look a the...

30 September 2020 3,642 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View