Hi Balaji.

You can try the spot culture growth inhibition assay (SPOTi). The compounds are dissolved in a suitable solvent at a concentration x1000 the one you want to test. Then you dispense your stock solution (2 uL) into the middle of each well of a 24 well plate. You then dispense 2 mL of the molten agar media (typically Middlebrook 7H10) into each well, mix well and let it solidify. After 1 hour, the bacterial cultures are diluted and 2 uL are put on top of the solid media in the middle of each well (around 500-1000 cells per well). The plates are incubated and direct visual reading enables MIC determination as the lowest concentration where no bacterial growth is visible. Please refer to:

http://www.ncbi.nlm.nih.gov/pubmed/20401596

http://www.ncbi.nlm.nih.gov/pubmed/21622974

http://bmjopen.bmj.com/content/3/6/e002672.full?rss=1

I appreciate the answer, thanks, but is 1000 cells per well enough? because in REMA i am adding some 15 lakhs per well. so i have a hitch whether this result (with SPOTi) matches to that of the REMA? (though i understand the concept that the latter is liquid culture)