My melanoma cell line doesn't grow well suddenly, May i know the possible reasons for that?

I have 92-1 of melanoma cell line , when i thawed them they were totally normal , but after first passage , the cells begun not growing well and not totally attaching on the bottom of the flask...

30 June 2020 3,622 4 View

How to calculate of total phenolic and total flavonoid content?

Dear all colleagues We keep working for long time on antioxidant and bioactive properties of plant extracts however we some time miscalculate the accurate values my question is as follow: We...

30 April 2020 8,760 6 View

Can I have an activated carbon with lower heavy metals removal efficiency than its Raw macroalgae?

Hello dear sir/Madam currently am working on producing activated carbon from red macroalgae by method of acid impregnation/pyrolsis. also am doing batch adsorption for lead Pb+2 amd Cu+2 for...

05 May 2019 6,989 9 View

How can Artificial Neural Networks (ANN) model be used by programming languages known that it is created by MATLAB ?

If ANN model is created using MATLAB 2015a or later. How this model is used in other programming languages e.g. Python, PHP or any. How can I pass parameters if the input of the ANN model is...

24 June 2018 6,710 4 View

What is the relation between pressure difference made by the fan and fan speed (or tip speed)?

Hello I'm trying to make a simulink model for Venturi tube, for the purpose of measuring volumetric airflow by making a pressure difference with a fan. The paper "Model-based analysis and...

13 December 2016 9,768 6 View

Is there a mathematical model that describes knowledge acquired by an individual?

If somebody wants to mathematically model data, information, and knowledge. Data represents a raw material for processing service delivery solutions to produce information. Knowledge acquired by...

01 January 1970 229 4 View

What your opinion about using avifavir for covid-19 treatment.?

Russian hospitals start receiving indigenously made anti-COVID drugs; mass production launched

01 January 1970 9,426 12 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

How can i perform logistic regression on nominal vs scale variables in SPSS?

Dear all, I would like to perform a logistic regression analysis on the correlation between surgery duration time represented in minutes (Scale independent variable) with rate of neuropraxia as...

14 February 2021 4,054 3 View

For acid treated montmorillonite clays what will be the result of surface area?

The organo montmorillonite nanoclays modified with octadecylamine were used for the experiment. After acid treatment, the surface area will be increase. However, in my experiment, the surface area...

10 February 2021 1,581 3 View