Can anyone help with old outer membrane protein clone not being expressed in BL-21 E. coli?

Prashant Sharma @Prashant_Sharma7

11 November 2013 0 5K Report

Some time RE is working some time it is not. What is the solution?

As expression is not working I did transformation again from Dh5 alfa.

Still it is not working.

Now I tried to re-clone it so the restriction is not working. I used BamHI, EcorI and SalI and XhoI.

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