Prashant Sharma

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Questions related from Prashant Sharma

Can anyone help with old outer membrane protein clone not being expressed in BL-21 E. coli?

Some time RE is working some time it is not. What is the solution? As expression is not working I did transformation again from Dh5 alfa. Still it is not working. Now I tried to re-clone it so the...

11 November 2013 4,996 0 View

How can we add polypeptide to DNA? Please tell me If someone has done it or if anyone has ideas.

Attaching any peptide or protein to DNA.

10 October 2013 5,736 1 View

I am cloning a 4400 base pair gene using pET-28a vector. After many attempts I am not successful. Please suggest how can I get a full gene.

I have also done it in two parts. For one part I got the clone, for part 2 I am not getting any result. I have also changed temperature parameters .

04 April 2013 6,200 7 View