Can anyone help with my problem with periplasmic protein extraction by osmotic shock?

Giggsy Matinny @Giggsy-Matinny

08 November 2015 1 1K Report

I'm very confuse about time for incubation of protein after add sucrose buffer and osmotic shock buffer(MgSO4).

and volume of each buffer were added?

Buchs Natasha

Maybe you can find some help in this article

http://onlinelibrary.wiley.com/doi/10.1021/bp034030s/pdf

Good luck

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