Question about plasmid digested by two-cut-site RE at MSC?

13 February 2016 1 414 Report

Here I have a basic question.

My cutting vector run faster than uncut when cut with MscI and XhoI.

Who can tell me it could happen in the normal situation?

It is normal scenario. The uncut plasmid will move slower than cut one. I have attached one picture you can compare this with your result.

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