1- Is the Fluorescence Resonance Energy Transfer (FRET)in the yeast different from the once in mammalian cells? I so, what is the difference?
2- Can I perform FRET to distinguish the interaction between 2 mammalian proteins by using yeast?If yes, why do many literatures report mammalian cells?
3- have bacteria been used to do FRET? and what is the difference from the above approach?
4-Should the target proteins that bound to fluorescent markers (CFP and YFP) be expressed on the cell surface to measure FRET?
I appreciate your time and thank you in advance