Hi friends, looks like I might need to look at some metabolites in exosomes floating around in blood. I need good suggestions for what to do/not do for isolating the exosomes. Some of the blood samples may be tiny, e.g. 50 uL.
Suggestions/advice = buy you a beer or a pizza if we run into each other!
why dont you visit me in my office across the road, then I can tell you how I have isolated them from serum :-) remember the pizza
I found an article in Proteomics Journal volume 13 issue 22 page 3354-3364 "Comparative proteomics evaluation of plasma exosome isolation techniques and assessment of the stability of exosomes in normal human blood plasma" I don't have the file though. So you need to get one yourself.
Thanks Narong! I really appreciate the help. Hadn't spotted that paper. Jens, I hope you like pepperoni and see you soon!!
Total exosome isolation reagent (from serum) or (from plasma) works well, on low vol as well
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