I am trying to conjugate peptides to polycaprolactone (PCL) using carbodiimide chemistry i.e. DCC (N.N'-dicyclohexylcarbodimide) /NHS (N-hydroxysuccinimide). I am thinking of using DMF (N,N-Dimethylformamide) or NMP (N-methyl-2-pyrrolidone) as a solvent. Many protocols suggest a dialysis step after the "carboxylic" activation in order to remove unreacted DCC and NHS (dialysis performed against excess of pure solvent). However, the "dialysis sacks" are made from cellulose and often harden on coming in contact with organic solvents (prior discussions on RG).
Can anyone suggest what material would be best suited for carrying out dialysis in an organic solvent? Any relevant article would also help. Thank you.
Dialysis tubing for laboratory use is typically made of a film of regenerated cellulose or cellulose ester. However instead of this you can use dialysis membranes made of polysulfone, polyethersulfone (PES), etched polycarbonate which are also available as substitute or try membrane filters of same materials.
Rahul S. Tade Thanks Rahul. Just like you have mentioned, I had started using tubes made of Regenerated cellulose some time back and it suits my application really well. Cellulose ester tubes sometimes create problem with organic solvents, like they Harden up sometimes preventing osmosis.
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