Srujan Singh

15 Questions 20 Answers 0 Followers

Questions related from Srujan Singh

Need help with determining linear equation and goodness of fit (R-squared) for a dataset with non normal distribution?

Hi RG community, I have a dataset which has a non-normal distribution and I want to perform linear regression on it (using Graphpad Prism 9.3.1). Here is the process I follow: 1. First determine...

03 October 2022 2,386 7 View

Can someone provide some suggestions related to DSC (differential scanning calorimetry) characterization of polycaprolactone undergoing degradation?

Hi, I am trying to subject a biodegradable polymer, Polycaprolactone (PCL, MW: 50,000 kDa) to enzymatic degradation for a period of 3 weeks and assess the extent of degradation by tracking the...

16 August 2021 3,770 1 View

Need help with quantifying pro-MMP2/MMP-2 from zymographs. Can someone help?

Hi, I am trying to study the expression profile of matrix metalloproteinase (MMP-2) from human mesenchymal stem cells (hMSCs) in an osteogenic pathway. I have conditioned media (CM) collected...

07 August 2019 3,339 2 View

Can somebody help with determination of %yield of product using NMR spectrum?

Hi All, I am synthesizing acrylated polycaprolactone (PCL-A) by reacting polycaprolactone (PCL) monomers (Mw: 50k Da) with acryloyl chloride in the presence of a non nucleophilic base i.e....

15 May 2019 2,022 3 View

Can DMAP (4-Dimethyl amino pyridine) be used instead of TEA (Triethylamine) as a base for acrylation of polyester diols?

I am trying to convert (modify) the terminal hydroxyl (-OH) groups of a polyester i.e. polycaprolactone-diol (PCL-diol) to acrylate group. I am planning to achieve this by reacting the PCL-diol...

09 April 2019 5,787 0 View

Can anyone help with dialysis for chemical synthesis (in organic solvent)?

I am trying to conjugate peptides to polycaprolactone (PCL) using carbodiimide chemistry i.e. DCC (N.N'-dicyclohexylcarbodimide) /NHS (N-hydroxysuccinimide). I am thinking of using DMF...

18 September 2018 3,748 2 View

What are the best characterization techniques one could use to determine the property of hydrogels in the wet state (soft materials in general)?

Currently I am working on preparing alginate based hydrogel (in the form of beads) to use it as a carrier for controlled drug release. I want to use them in their pristine form i.e. the wet state...

16 May 2016 4,716 4 View

Can somebody help with correlating BET isotherm and pore distribution data?

I have prepared a Carbon/Silica composite and have done its BET analysis and pore size distribution analysis. However my BET isotherm shows Type IV nature which indicates mesoporosity however the...

10 April 2015 7,287 2 View

Can someone please tell me the "image file specifications" of a plot exported from origin that can be used for a manuscript?

I am trying to export some plots from Origin (Version 9.1) and then form a collage out of these plots and then use in a word file. I am using the following specifications: .tiff format, 600 dpi, 8...

17 February 2015 8,624 1 View

How does one go about analyzing XPS peaks ?

I am new to XPS technique. Lately I have been trying to analyze some XPS peaks of my samples. How I do it is: First I plot the "narrow" scan of an element (peak) in Origin, then using Multiple...

05 February 2015 4,366 11 View

How can I decide the degassing temperature (and the duration for degassing process) for doing the BET analysis of a sample?

I am doing the BET analysis of my samples and observing that the constant "c" is coming out to be negative, though it gives a value of surface area and also the starting point of the adsorption...

30 December 2014 5,588 42 View

What's the basic difference between: Aerogel, Xerogel, Cryogel and Hydrogels?

I wanted to know how to qualify/classify a substance as an aerogel or xerogel etc. Is it solely the way in which it is synthesized or is it the final properties of the substance that is...

30 October 2014 5,828 26 View

How do you measure the surface area of samples in the form of monoliths?

I want to find out the surface area of my samples, but they are in the form of monoliths. For doing BET measurements, I have to crush the sample into powdered form using pestle and mortar. Is that...

08 October 2014 4,674 9 View

How do I separate silica from a homogeneous solid (monolith) of Silica + Zinc Oxide?

Consider monoliths with homogeneous composition of Carbon(C), Silica(SiO2) and zinc Oxide(ZnO). Calcining them and obtain monoliths of (SiO2 +ZnO). Now, how to separate ZnO from silica? ( if HF...

04 October 2014 8,688 4 View

What is the formula for measuring conductivity of a small slab of sample (of uniform thickness) using four probe method?

I have some (carbonaceous material) samples which are in the shape of slabs with uniform thickness. What formula should I use to measure it's conductivity if I am using four probe method?

28 September 2014 9,334 7 View