20 Questions 21 Answers 0 Followers
Questions related from Srujan Singh
Dear RG Community, I am reaching out to see what techniques do you use/recommend to validate your gene expression trends obtained through spatial transcriptomics (ST) technology (10x Visium)?...
15 July 2025 3,213 0 View
Dear Community, I want to perform automated segmentation of histological whole slide images (WSIs) of kidney tissue to extract morphological features associated with various structural units like...
27 May 2025 7,923 1 View
Hi RG community, I have a dataset which has a non-normal distribution and I want to perform linear regression on it (using Graphpad Prism 9.3.1). Here is the process I follow: 1. First determine...
03 October 2022 2,444 7 View
Hi, I am trying to subject a biodegradable polymer, Polycaprolactone (PCL, MW: 50,000 kDa) to enzymatic degradation for a period of 3 weeks and assess the extent of degradation by tracking the...
16 August 2021 3,816 1 View
Hi, I am trying to study the expression profile of matrix metalloproteinase (MMP-2) from human mesenchymal stem cells (hMSCs) in an osteogenic pathway. I have conditioned media (CM) collected...
07 August 2019 3,386 2 View
Hi All, I am synthesizing acrylated polycaprolactone (PCL-A) by reacting polycaprolactone (PCL) monomers (Mw: 50k Da) with acryloyl chloride in the presence of a non nucleophilic base i.e....
15 May 2019 2,067 3 View
I am trying to convert (modify) the terminal hydroxyl (-OH) groups of a polyester i.e. polycaprolactone-diol (PCL-diol) to acrylate group. I am planning to achieve this by reacting the PCL-diol...
09 April 2019 5,837 0 View
Hi, I want to modify (conjugate some molecules) the backbone of Polycaprolactone-diols (PCL-diols) using Michael Addition reaction. Once the conjugation is done, I would like to 3d print the...
06 April 2019 2,169 0 View
I am trying to conjugate peptides to polycaprolactone (PCL) using carbodiimide chemistry i.e. DCC (N.N'-dicyclohexylcarbodimide) /NHS (N-hydroxysuccinimide). I am thinking of using DMF...
18 September 2018 3,791 2 View
Currently I am working on preparing alginate based hydrogel (in the form of beads) to use it as a carrier for controlled drug release. I want to use them in their pristine form i.e. the wet state...
16 May 2016 4,758 4 View
I am trying to prepare polymeric microcapsules via. a W/O/O emulsion synthesis process. So my technique essentially begins with formation of a W/O1 emulsion system formed by homogenization where...
26 October 2015 1,865 6 View
I have prepared a Carbon/Silica composite and have done its BET analysis and pore size distribution analysis. However my BET isotherm shows Type IV nature which indicates mesoporosity however the...
10 April 2015 7,342 2 View
I am trying to export some plots from Origin (Version 9.1) and then form a collage out of these plots and then use in a word file. I am using the following specifications: .tiff format, 600 dpi, 8...
17 February 2015 8,661 1 View
I am new to XPS technique. Lately I have been trying to analyze some XPS peaks of my samples. How I do it is: First I plot the "narrow" scan of an element (peak) in Origin, then using Multiple...
05 February 2015 4,431 11 View
When it comes to material property there are many terms like toughness, stiffness, resilience, hardness,elasticity etc. So what does one compare between two samples in order to determine the...
18 January 2015 8,545 2 View
I am doing the BET analysis of my samples and observing that the constant "c" is coming out to be negative, though it gives a value of surface area and also the starting point of the adsorption...
30 December 2014 5,675 42 View
I wanted to know how to qualify/classify a substance as an aerogel or xerogel etc. Is it solely the way in which it is synthesized or is it the final properties of the substance that is...
30 October 2014 5,916 26 View
I want to find out the surface area of my samples, but they are in the form of monoliths. For doing BET measurements, I have to crush the sample into powdered form using pestle and mortar. Is that...
08 October 2014 4,721 9 View
Consider monoliths with homogeneous composition of Carbon(C), Silica(SiO2) and zinc Oxide(ZnO). Calcining them and obtain monoliths of (SiO2 +ZnO). Now, how to separate ZnO from silica? ( if HF...
04 October 2014 8,736 4 View
I have some (carbonaceous material) samples which are in the shape of slabs with uniform thickness. What formula should I use to measure it's conductivity if I am using four probe method?
28 September 2014 9,369 7 View
