I would very appreciate any suggestions about primers for cytochrome b, that work well on Trichoptera samples. We used some primers from Simon et al 1994, but with very low success. The template DNA should be fine because for example cox1 amplifies very well.
Hi Nadine, I would need primers that should work with Trichoptera samples, amplifying cytochrome b. We used ones described in Barrclough et al 1999 but they did not work.
Why do you need Cytochrome B? Can't you use other highly conserved regions? Again my question: What is your goal? What will you do with your amplified cytochrome b samples?
Well, we discovered, in other cooccuring insect species, that cytochrome b could be a good marker for population genetic studies. And so we would like to use it also for other species, like caddis flies for example.
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