Can anyone help with a scratch wound healing assay?

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I have been getting inconsistent results with my immunoprecipitation experiments. What am I possibly missing?

It goes to an extent that while one trial gives good results (positive control coming down with bait while negative control does not), another trial shows me that none of my prey proteins are in...

02 March 2016 8,452 13 View

What is the nucleotide intake rate of bacteria in the gut?

Lateral gene transfer phenomenon is an important factor that should be considered during the utilization of GMOs as food sources since the transfer of antibiotic or pesticide resistance genes to...

02 March 2015 4,181 2 View

How to check plagiarism?

I'm a senior undergraduate student and I've started writing my thesis. I'm trying to be careful to avoid plagiarism but i couldn't find a trustworthy plagiarism checker on internet, there are some...

02 March 2015 6,196 5 View

What is the importance of the host organism with which the secondary antibody is synthesized?

I'm searching for a secondary antibody which I'll use with a primary Rac1 synthesized in rabbit. But what is the importance of the host organism since the secondary antibody will be synthesized by...

09 October 2014 8,633 5 View

Is Cytoskeleton, Inc a trustworthy source for purchasing Rac1 antibody?

It would be great if you could share with me where you got your Rac1 antibody too (if you used any).

09 October 2014 1,274 6 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended?

In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...

05 August 2024 8,056 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View