I have been getting inconsistent results with my immunoprecipitation experiments. What am I possibly missing?

It goes to an extent that while one trial gives good results (positive control coming down with bait while negative control does not), another trial shows me that none of my prey proteins are in...

02 March 2016 8,340 13 View

What is the nucleotide intake rate of bacteria in the gut?

Lateral gene transfer phenomenon is an important factor that should be considered during the utilization of GMOs as food sources since the transfer of antibiotic or pesticide resistance genes to...

02 March 2015 4,074 2 View

How to check plagiarism?

I'm a senior undergraduate student and I've started writing my thesis. I'm trying to be careful to avoid plagiarism but i couldn't find a trustworthy plagiarism checker on internet, there are some...

02 March 2015 6,110 5 View

What is the importance of the host organism with which the secondary antibody is synthesized?

I'm searching for a secondary antibody which I'll use with a primary Rac1 synthesized in rabbit. But what is the importance of the host organism since the secondary antibody will be synthesized by...

09 October 2014 8,555 5 View

Is Cytoskeleton, Inc a trustworthy source for purchasing Rac1 antibody?

09 October 2014 1,197 6 View

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02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View