Does Anyone Know of a Way to Detect Melatonin that is NOT HPLC, RIA or Mass Spec?

I tried to do a Slot Blot and was not successful and melatonin is only .23 Kda so it would probably run off a western blot. Has anyone had success with other methods?

23 January 2021 1,388 3 View

How to quantify total protein in crooked western lanes?

I've been using Revert700 total protein stain to detect total protein in my western lanes. While this stain is robust & reversible, I have trouble quantifying in Image Studio Lite (LiCor) and...

05 December 2020 9,014 3 View

Any advice or articles on combining one qualitative approach with the methods of a different approach?

I am a doctoral student and I am looking to do a qualitative research study that looks at the potential influence of teachers on the way minority students view their leadership capabilities....

28 November 2020 6,217 5 View

Troubles cloning - correct RE digestion and then incorrect sequencing?

I am inserting an MPP5 gene insert into a pCAGGs backbone. When I am finished with the mini prep, I find that my RE digestion is correct, but then when I send it to be sequenced, the MPP5 gene is...

23 November 2020 7,962 2 View

How to convert a cif file to a qe file?

I am trying to convert a cif file to a Quantum espresso file to do DFT calculations

10 September 2020 1,535 4 View

What are the available Nanocomposite Databases?

I am looking for data files of Nano Composites. Any form of data, be it XRD outputs, SEM or TEM images or anything. Where can I find them?

28 August 2020 9,927 5 View

Can I degas drug solutions before applying to mammalian cells for electrophysiology?

I am applying different drug solutions to cells that I am whole-cell patching. Each solution is in a syringe connected to a thin tube that goes through a barrel manifold into a glass micropipette...

24 August 2020 1,956 2 View

How to count % GFP positive cells and GFP intensity using imagej?

I have infected BHK21 cells with a virus that expresses GFP. I want to count the percentage of GFP positive cells and the intensity of the GFP. How do I do this with fluorescent microscope...

17 August 2020 4,009 2 View

CD8+ T cell and natural killer isolation kits from rat spleen?

Hello, Does anyone have any experience with isolation of CD8+ cytotoxic T cells and natural killer cells from rat spleen? I'd prefer kit-based if possible, but any protocols you can provide would...

30 July 2020 5,387 6 View

Best ways to analyze crowdsourced data for a qualitative study?

Hello, I am currently working on a project analyzing crowdsourced data from online trip reports and social media applications. However, I am struggling in defining the methodology for this...

25 February 2020 4,401 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

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01 March 2021 3,905 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View