After oxidation of tannins, the oxidized product when used in a chemiluminescence assay (Ox-polyphenol + buffer pH >8) present for a short time (sec) increased luminiscence.
It is well known that ion species such as Fe2+, Cu+ could form tannin-metal complex material, in this way due to the complex formation, should be a decrease in luminiscence in comparasion with blank sample(without metal), due to less free Ox-polyphenol quantity remained in the model system. But this theory could be applyed only for 0.001mg/L - 0.05mg/L. At higher concentration (>0.05mg/L) of these metals, for Cu+ there is no difference in luminescence (ex. photon count value for 0.07, 0.1 mg/L is the same as for 0.05mg/L). The same effect could be observed for Fe2+.
Why at higher than 0.05mg/L (0.1mg/L, 0.5mg/L) there is not being observed any further step in decrease of luminescence while increasing metal concentration?
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