Can anyone help to find a PBI membrane supplier?

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SH-SY5Y total cholesterol?

I'm currently doing total cholesterol assay in SH-SY5Y cells treated with 0, 25, 50, 100ug cholesterol/mL (using cholesterol:mbcd, sigma) Lipid extraction is done by chloroform and methanol. After...

12 April 2024 9,353 3 View

What is the reason for double positive signal clouds in ddPCR?

I am testing a methylation-specific ddPCR assay and observed two positive droplet layers on the FAM channel. Based on the 2D group it does not seem to be affected by the signal from HEX channel....

13 February 2024 4,060 1 View

A biacore 3000 error code show up, any way to remove the error code?

A error code related to biacore 3000 autosampler showed up after I ran a new template and the needle was inserted into wrong position while filling sample. The needle still works fine during...

06 February 2024 1,748 0 View

What cause GGBS to replace cement up to 70% when comparing with PFA?

Is it because of composition difference? I am doing the literature review. It looks like that both GGBS and PFA would react with calcium hydroxide and water to form CSH gel. But then why GGBS...

05 February 2024 1,493 3 View

Why do I get inverted signal in Streptavidin-Horseradish Peroxidase /Luminol detection during LightShift Chemiluminescent RNA EMSA kit?

I am doing EMSA using LightShift Chemiluminescent RNA EMSA Kit from Thermo Scientific. For the detection, I use stabilized Streptavidin-Horseradish Peroxidase Conjugate and Chemiluminescent...

30 January 2024 2,314 1 View

Transparent spots on hematoxylin & dab stained slices?

I was performing IHC on paraffin embedded mice vascular tissue ,after examining the sections under microscope,I found irregular transparent spots on top of my tissue section, please help,thank you.

22 January 2024 2,789 3 View

Can I Get Help with a Challenging Research Survey?

Hello, I am a researcher from Korea working on a study about traffic accident perpetrators. I am encountering difficulties in conducting a survey among traffic accident perpetrators in Japan and...

31 December 2023 2,625 0 View

I use 0.05% EDTA-trypsin to detach Hep G2 cells, and I culture them in DMEM(HG) with 10% FBS, 1% p/s, and 1(NEAA),but my cell will clump?

i use 0.05% EDTA-trypsin to detach Hep G2 cells 6min then neutralize the trypsin- EDTA with DMEM(HG) with 10% FBS, 1% p/s, and 1(NEAA) and centrifuge gently then resuspend cells in fresh...

10 December 2023 383 5 View

SH-SY5Y seeding density?

I'm working on SH-SY5Y viability test with MTT assay, using vitamin C and H2O2. In our lab protocol, after 30-48h from cell seeding it reaches 70% confluency and gets ready for cell deprivation....

03 October 2023 9,458 3 View

Ros determination method?

Can zebrafish use HPLC or GC to detect ros? If so, how can it be detected?

20 September 2023 6,157 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View