What is the cause of a smear from direct DNA amplification from human buccal swab samples ?

Dear fellow scientists, I like to performed a direct amplification of a fragment of 222-bp DNA from human genome and use is for further analysis. By using the following methods, I obtained...

07 February 2021 891 3 View

How to edit the coloring in Geneland output?

As the original published paper of Geneland (back in 2005) and most of published papers suggested, the diagrams of posterior probabilities of population membership should group the closely related...

31 August 2020 8,073 1 View

Can Niobium tube act as a protective casing for sintering powder alloy sample?

Hi, as a rookie in powder metallurgy field, I've heard that for sintering CP Ti Powder at 1200C in tube furnace, niobium tube or molybdenum crucible would be used as protective casing instead of...

12 January 2020 1,501 12 View

Company that can make pre-alloyed powder?

Hi, I am doing a additive manufacture of high entropy alloy research. Do you guys know any company that make customized pre-alloyed powder? I've checked with several companies and they only sell...

25 November 2019 9,335 8 View

How to identify the residues that have interaction from autodock or other docking website results?

28 August 2019 655 2 View

Endogenous treatment effect with two endogenous variables?

Hi, I would like to estimate the impact of formal microcredit and informal microcredit on consumption as C = b1X1 + b2X2 + b3M + b4E + ... + e, where X1, X2 are exogenous variables; M is total...

06 September 2018 8,723 4 View

HEK cells coming off after medium change?

19 September 2017 373 4 View

Country Image and Firm Image?

24 December 2016 7,923 1 View

In logistic regression, is a linear relationship always nonlinear if the predictor variable is not limited?

Hi guys, As I am analyzing my data and testing a nonlinear relationship hypothesis between the variables, an interesting question came to my mind which I don't have enough expertise (or I didn't...

01 November 2016 7,759 4 View

Is there anything wrong to separate two groups according to the vertex under a quadratic logistic regression?

Hi all thank you for your attention I have used logistic regression to test whether a predictor and it's squared form would predict a binary outcome. Now that both variables are significant and...

18 October 2016 7,565 2 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View