I am trying to isolate heavy metal resistant Potassium solubilising plant growth promoting rhizobacteria which can promote plant growth during heavy metal stress conditions.
Luria Bertani (LB) agar with heavy metal added was usually used for microbiological isolation, but these materilas should be sterilized separately before mixing .
Maybe, you can refer to this highlight paper :
Dell’Amico, E., Cavalca, L. and Andreoni, V. 2005. Analysis of rhizobacterial communities in perennial Graminaceae from polluted water meadow soil, and screening of metal-resistant, potentially plant growth-promoting bacteria. FEMS Microbiol. Ecol., 52: 153–162.