Can anyone help me with primer design and predicted sequences?

07 April 2014 1 6K Report

I wanted to design primers for my favorable mRNA (for real time) but for this mRNA in NCBI, there are several predicted transcript variants, in addition to the main mRNA sequencing. Main mRNA sequencing contains only 100 bp different nucleotides than predicted transcript variants. Should I design primer for these 100 bp nucleotides or is it not important to design primer for common sequences?

Shrey Kohli

It depends if the transcript variants are affected by the treatment you want to do.. Mostly its not a problem.. But if your transcript variants are quite different in function, then you need to be careful.. In this case, it would be good to design only for the different sequences

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