Presently I'm working on use of SNP in genetic diversity in maize. Is there anyone with usefull information are highly needed and welcome? Plant breeders, biotechnologist.
Hi,
Maybe you can try web resources at CIMMYT. I for one have not used any techniques for SNP analysis.
There are protocols though based on the imperfect alignment at the polymorphic site (mismatch) and nuclease-mediated fragmenting of the DNA using a Celery or Mungbean nuclease, gel electrophoresis and sequencing of resultant fragments.
This paper is taken as the bible of SNPs
http://nar.oxfordjournals.org/content/32/8/2632.full
Best Wishes
Dilantha
Next-generation sequencing (NGS) technologies have been recently used for whole genome sequencing and for re-sequencing projects where the genomes of several specimens are sequenced to discover large numbers of single nucleotide polymorphisms (SNPs) for exploring within-species diversity. There are many approach that you can use.
Hello,
I see that one of my publications was mentioned. As mentioned, there are many different techniques you can use to discover nucleotide variation. These include lower-cost enzymatic methods where you would interrogate PCR amplicons in gene regions of choice all the way to sequencing large portions of a genome (I don't think you'll be sequencing whole maize genomes). It depends on what your question is and what sort of budget and infrastruture (e.g. bioinformatics) you have. If you want to try some low cost methods to look at SNPs in PCR amplified genomic regions, please email me at [email protected]. My group has developed a series of easy to use protocols. We can also arrange to send you some materials to help you optimize your protocols.
Best regards,
Brad
Dear Omikunle
I hope this link will be useful for you :
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0028334
Good Luck
- Badges
- Science topic